Biochem/physiol Actions

Reversible: yes

Product does not compete with ATP.

Primary TargetMreB

Cell permeable: yes

General description

A cell-permeable isothiourea compound that specifically, rapidly and reversibly perturbs MreB function without affecting eukaryotic actin polymerization. Reported to affect the growth and morphology of Caulobacter crescentus, convert rod-shaped E. coli to round cells, and block DNA segregation without affecting DNA replication. Suggested to interact with MreB′s nucleotide binding pocket and is useful for the temporal analysis of MreB function.

A cell-permeable isothiourea compound that specifically, rapidly, and reversibly perturbs MreB function without affecting eukaryotic actin polymerization. Reported to affect the growth and morphology of Caulobacter crescentus, convert rod-shaped E. coli to round cells, and block DNA segregation without affecting DNA replication. Suggested to interact with the nucleotide binding pocket of MreB and is useful for the temporal analysis of MreB function.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gitai, Z., et al. 2005. Cell120, 329.Jensen, R.B., et al. 2002. Nat. Rev. Mol. Cell Biol.3, 167.Iwai, N., et al. 2002. Biosci. Biotechnol. Biochem.66, 2658.

Packaging

Packaged under inert gas

25 mg in Plastic ampoule

Reconstitution

Following reconstitution in DMSO, aliquot and freeze (-20°C. DMSO stock solutions are stable for up to 1 month at -20°C. Reconstitute in H₂O just prior to use; aqueous stock solutions are stable for up to 12 hr. Stable for up to 2 days in acidic medium.

Warning

Toxicity: Standard Handling (A)